B.Pharm Lab. Instruction Manuals

Pharmacology I

APHE Anatomy, Physiology, and Health Education

Pharmaceutical Analysis

Pharmacy study material

Bones and Skeleton System

Bone disease (Gout) (Rheumatoid arthritis) (Osteoarthritis) (Osteoporosis)

Cancer and music therapy

Memory of water

1.2.5 Anatomy Physiology and Health Education I

clotting time

Lab 1 Determination of clotting time with the help of Wrighting's method


To determine the clotting time of the patient.


Normally blood remains in the blood vessels in liquid form. When it is drawn from the body it becomes into semisolid gel. The mechanism of formation of gel is called blood clotting or coagulation. Blood does not clot inside vessels due to presence of heparin and anti thrombin III. A blood clot is a gel that contains the formed elements of the blood, arrested in the stable fibrin mesh.

The factors, involved in blood coagulation are: Fibrinogen (I), Prothrombin (II), Thromboplastin (III), Calcium (Ca++, IV), Labile Factor (V), Stable Factor (VII), Anti-Hemophilic Factor A (VIII), Anti-Hemophilic Factor B (IX), Stuart Power Factor (X), Anti-Hemophilic Factor C (XI), Hageman Factor (XII), Fibrin Stabilizing Factor (XIII). Blood plasma without clotting factors is called serum.

Factors III, IV and V are synthesized in the platelets of plasma. Factors I, II, VII, VIII, IX, X, XI, and XII are synthesized in liver. Factor XII can be synthesized in liver and platelets. Factor IV is also obtained from diet and bones.

Pathway of blood coagulation: Extrinsic pathway (Tissue damage)


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