1.2.5 Anatomy, Physiology, and Health Education I Laboratory
Lab 6 Total Leukocyte Count with improved Neubauer's chamber
Developed by Prova Biswas
Principle: The blood specimen is diluted 1:20 in a WBC pipette with the diluting
fluid (water: glacial acetic acid: gentian violet = 97:2:1) and the cells are counted under low power of the microscope
(10X) by using a counting chamber. The glacial acetic acid lyses the red cells while the gentian violet slightly stains
the nuclei of the leukocytes to locate the WBC under microscope.
Theory: White blood cells, present in plasma take part in body defense against invading
micro-organisms. They are produced from the pluripotent stem cell in the bone marrow in adults. In case of foetus
haemopoiesis occurs in liver and spleen.
Table 1. Normal ranges of total leukocyte count:
Table 2. Differential leukocyte count:
Clinical Significances of total leukocyte count: Increase in total leukocyte count of
more than 10,000/cu mm (µl) is known as leukocytosis and decrease of less than 4 000 cu mm (µl) as leukopenia.
Causes of leukocytosis: i. it is common for a transient period in infections
(bacterial, protozoal (malaria), or parasitic), ii. leukocytosis is also observed in severe hemorrhage and in leukemia
ii. high temperature iii. severe pain iv. accidental brain damage.
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