3.5.3 Pharmacology I
Lab 6 Evaluation the action of Xylocaine on Rabbit's skin
Xylocaine (Lidocaine/ Lignocaine), an amide derivative,
acts as a local anesthetics, available as bolus injection (5, 10, 15, 20, 40, 100 & 200 mg/mL),
jelly (2% w/v), ointment (2.5%, 5% w/v), parenteral injection (0.5%, 1%, 1.5%, 2%, & 4% w/v), premixed
solution (2, 4 & 8 mg/mL in D5W), spray (10% w/v), topical solution (2% & 4% w/v), cream (0.5%), liquid (2.5% w/v)
and gel (0.5%, 2.5% w/v).
Xylocaine is used as ventricular anti arrhythmic (50-100 mg bolus i.v. given at 25-50 mg/ mins), local anesthetics (2-5% solution or ointment or 15 ml of xylocaine viscous q 3-4 hrs
to oral or nasal mucosa), procedure anesthetic (lidocaine HCL, with NA 7 mg/ kg., should not exceed 500 mg. total,
without NA, 4.4 mg/ kg., should not exceed 300 mg. total) and in pain due to burning or itching.
Onset of action
Onset of lidocaine by topical route is 30-60 seconds and duration of action is variable.
A healthy Rabbit, Lidocaine solution (2% w/v), scissor, hair removal cream, Spirit, needle, syringe, cotton.
Hairs of dorsal surface of a Rabbit is removed with the help of a scissor and finally cleaned with a hair removing cream.
Response of the animal is noted by pricking on the cleaned surface before the injection of the drug. Lidocaine (2 % w/v, 0.5 ml)
is injected (intra dermal) on the cleaned surface in the junction between Markel layer of the epidermis and dermis. The time of
injection is noted. Swelled area in the skin is marked immediately after injection. Animal's response is observed in every 10 mins
till the response come back on the injected surface.
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