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Pharmacology I

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Bones and Skeleton System

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3.5.3 Pharmacology I

pills

Lab 9 Determination of anti-oedemic action of a NSAIDs (Aspirin) with the help of Plethysmometer (Cont'd...)

Materials:

Healthy Wister rats (80-120 gm),

Plethysmometer, needle (26 G), syringe, pan balance, marker pen,

Aspirin (100 mg/kg), carrageenin (1% w/v in normal saline), distilled water, picric acid.

Procedure

The anti-inflammatory activity of aspirin was evaluated against carrageenin induced paw edema in rats. Wister rats were divided into two groups containing six animals in each group. A suspension of carrageenin was prepared fresh just 1 hour before use and injected in the right hind paw(0.1ml/100 gm) of the rats of each group, following the oral or intraperitoneal administration of aspirin (100 mg/kg) or distilled water (0.1ml/100 gm) to test and control group respectively, 30 minutes before the carrageenin injection. The mean increase in paw volume, due to carrageenin induced edema was measured by the method of [Bhatt et. al., 1977] at zero hour (before drug and distilled water administration), and each hour interval up to 6th hour after carrageenin treatment [Winter et. al., 1963]. The percentage inhibition of the paw volume of test group was calculated and compared with the control group.

Reference:

[Bhat et. al., 1977] Bhatt K R, Mehta R K, Shrivastava, P N, A simple method for recording anti-inflammatory effect on rat-paw edema, Indian Journal of Physiology and Pharmacology, vol. 21, pp. 399-400, 1977.
[Winter et. al., 1963] Winter, C A, Risley E A, Nuss G, Carrageenan-induced edema in hind-paw of the rat as an assay for anti-inflammatory drugs, Journal of Pharmacology and Experimental Therapeutics, vol. 141, pp. 369-373, 1963.

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