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Pharmacology I

APHE Anatomy, Physiology, and Health Education

Pharmaceutical Analysis

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Bones and Skeleton System

Bone disease (Gout) (Rheumatoid arthritis) (Osteoarthritis) (Osteoporosis)

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Memory of water

3.5.3 Pharmacology I

swiss albino rat

Lab 8 Determination of the prolongation action of Diazepam on Pentobarbitone induced sleeping time (cont'd...)

Method

Animals are divided into two group (n=6). After weighing the animals, normal saline (0.1 ml/g) and diazepam were injected to group I and group II respectively. Time of injection is noted. After 20 minutes of injection both group of animals were given pentobarbitone (i. p.). Time of injection and time of sleeping were noted for individual animal. Thereafter the time of awaking is noted for individual animals of each group. The sleeping time is calculated for each group. Then mean, standard deviation, standard error and P value is calculated. The P value is calculated to analyze the significance of the results.

Result

Table 1 Effect of Diazepam on Pentobarbitone induced sleeping time

Animals no. Sleeping time (minutes)
Pentobarbitone+NS Pentobarbitone+Diazepam2
1
2
3
4
5
6
Mean
SD
SE
P value

Conclusion

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